Fig. 1: Preparation and optimization of COL/NIC-mPEG-PLGA-NPs.

a Preparation of nanoparticles using the double emulsification method. b–d The response surface model demonstrated the influence of mPEG-PLGA concentration, volume ratio of dichloromethane to acetone, and PVA concentration on nanoparticles NIC-EE (b), COL-EE (c), and size (d). e Effect of different lyoprotectants on COL/NIC-mPEG-PLGA-NPs.