Fig. 2: Chimeric SPPL proteases. | Communications Biology

Fig. 2: Chimeric SPPL proteases.

From: The N-terminal PA domains of signal-peptide-peptidase-like 2 (SPPL2) proteases impact on TNFα cleavage

Fig. 2: Chimeric SPPL proteases.The alternative text for this image may have been generated using AI.

a Schematic representation of SPPL family members and chimeric proteases. Protein domains of SPPL2a are depicted in green, those of SPPL2b in blue, SPPL2c protein domains in orange and of SPPL3 in red. Predicted N-glycosylation sites are marked with black tree-like structures and the HA-tag as black boxes. b Expression of SPPL family members and chimeric proteases. SPPL2a wt (2a wt), SPPL2b wt (2b wt) and all chimeric proteases were stably expressed under a doxycycline inducible promotor in dKO cells. Proteins were visualized using the anti-HA antibody 3F10. Calnexin was used as loading control.

Back to article page