Fig. 3: Subcellular localization of chimeric proteases. | Communications Biology

Fig. 3: Subcellular localization of chimeric proteases.

From: The N-terminal PA domains of signal-peptide-peptidase-like 2 (SPPL2) proteases impact on TNFα cleavage

Fig. 3: Subcellular localization of chimeric proteases.The alternative text for this image may have been generated using AI.

Wt and chimeric proteases were visualized using 3F10-fluorescein targeting the C-terminal HA-tag. For labeling of lysosomes Lamp1 (D2D11) was used, actin staining (phalloidin) served as plasma membrane marker, BiP for visualization of the ER and DAPI for nuclear staining. Nuclei are depicted in blue, SPPL wt and chimeric proteases in green and cell compartments in red. White bar = 10 μm. a SPPL2a wt and all SPPL2a chimeric proteases colocalized with the lysosomal marker. b SPPL2b wt and the SPPL2a/2b chimera colocalized with the plasma membrane marker, while the other chimera are not observed at the plasma membrane. c, d SPPL2c/2b and SPPL3/2b mainly colocalize with the ER marker.

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