Fig. 6: Kinetics of TNFα turnover by SPPL2a chimeras. | Communications Biology

Fig. 6: Kinetics of TNFα turnover by SPPL2a chimeras.

From: The N-terminal PA domains of signal-peptide-peptidase-like 2 (SPPL2) proteases impact on TNFα cleavage

Fig. 6: Kinetics of TNFα turnover by SPPL2a chimeras.The alternative text for this image may have been generated using AI.

a–c DKO cells (ctrl.) and dKO cells stably overexpressing either SPPL2a wt or the indicated chimeras were transiently transfected with full length TNFα (TNFα FL). Membrane isolates were incubated shaking at 37 °C for the indicated time periods and TNFα species were analyzed on Western Blot using FlagM2 antibody. Calnexin served as loading control. d The TNFα NTF-reduction over time is represented as absolute slope/ExF (calculation shown in Suppl. Figure 5b). Expression differences between chimeric and wt proteases are accounted for by division with the ExF at time point 0 min. Resulting values were all normalized to the SPPL2a wt protease sample. Mean + SEM, unpaired, two-tailed one sample t-tests of log-transformed (log2) values. ns=not significant, *p < 0.05, **p < 0.01 (p of 2b/2a = 0.0262, p of 2c/2a = 0.0048, p of 3/2a = 0.2336), n = 3–5 independent experiments (a: n = 4, b: n = 3, c: n = 5). The ExF is the mean (a: n = 4, b: n = 3, c: n = 5) of the ratio between the expression of the individual chimera and SPPL2a wt.

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