Fig. 8: Cleavage sites of chimeric proteases. | Communications Biology

Fig. 8: Cleavage sites of chimeric proteases.

From: The N-terminal PA domains of signal-peptide-peptidase-like 2 (SPPL2) proteases impact on TNFα cleavage

Fig. 8: Cleavage sites of chimeric proteases.The alternative text for this image may have been generated using AI.

a Amino acid sequence (single letter code) and schematic representation of the TNFα N-terminus. Arrows indicate the known SPPL2 cleavage sites39. b, c Mass spectrometric analysis of TNFα ICDs generated from either SPPL2a (b) or SPPL2b chimeric proteases (c) compared to the respective wt protease. Membranes from dKO cells co-expressing wt or chimeric SPPL proteases and TNFα FL were isolated and incubated for 30 min at 37 °C. TNFα species were isolated and analyzed by MALDI-TOF. Numbers indicate the position of the most C-terminal amino acid of the respective cleavage product as indicated in (a). Control spectra are shown in Suppl. Figure 6.

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