Fig. 3: Characteristics of monocytes and PD-1/PD-L1 signaling in PJI.
From: The role of PD-1/PD-L1 in overshooting osteoclastogenesis in periprosthetic joint infections
A Concentration of sPD-L1 and sPD-1 in serum as determined by ELISA. B Gene expression of PDCD1 in femoral bone. C, E Flow cytometry of PBMCs: prevalence of monocytes and PD-1 expression. Monocytes were gated using CD33 and CD14 markers. D, E Flow cytometry using bone marrow specimens: prevalence of monocytes and PD-1 expression. CD45-positive cells were gated first to exclude mesenchymal cells, followed by gating with CD33 and CD14 to identify monocytes. F, G Quantification of monocyte occurrence and PD-1 expression in PBMCs and bone marrow.
