Fig. 4: In vitro osteoclast stimulation indicates the role of PD-1 signaling.
From: The role of PD-1/PD-L1 in overshooting osteoclastogenesis in periprosthetic joint infections
A CCK-8 test for gradient concentration of PD-L1 and PD-1 inhibitor nivolumab for monocytes at time point 24 h, 48 h, and 72 h. B Representative overview of osteoclast generation with PD-L1 and/or nivolumab from monocytes of patients at explantation surgery in bright field. C Representative immunofluorescent images of monocytic osteoclast differentiation. Scale bar: 200 µm. D, E) Histomorphometric quantification of osteoclast number and % surface cover.
