Fig. 2: Validation of hiPSC-CM purity after CM-AI processing of two separate cell lines by flow cytometry. | Communications Biology

Fig. 2: Validation of hiPSC-CM purity after CM-AI processing of two separate cell lines by flow cytometry.

From: AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing

Fig. 2

a Flow cytometry plots of GFP + TTN-GFP hiPSC-CMs following CM-AI purification stained for cardiac troponin T (cTnT-APC). b Flow cytometry of untagged 19.9.11 hiPSC-CMs stained with cTnT-APC following CM-AI purification. c Summary of cTnT+ cell percentages from three independent differentiations using TTN-GFP and 19.9.11 hiPSC lines (n = 3 per line). Average purity was 92.0 ± 1.0% and 94.3 ± 7.2%, respectively. No residual expression of pluripotency markers (NANOG, POU5F1) detected post-CM-AI processing (qRT-PCR; Supplementary Table 1). Data are mean ± s.d.

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