Fig. 3: The K105 and T237 sites of dTSSK2 are essential for sperm motility. | Communications Biology

Fig. 3: The K105 and T237 sites of dTSSK2 are essential for sperm motility.

From: Testis-specific serine/threonine kinase dTSSK2 regulates sperm motility and male fertility in Drosophila

Fig. 3

a WB analysis showing expression in testicular of Flag-dTSSK2, Flag-dTSSK2K105M, Flag-dTSSK2T237A and wild-type flies. H3 was used as a loading control. b Qualitative fertility assay of wild-type (w1118), P{dTSSK2K105M}; dTSSK2−/− and P{dTSSK2T237A}; dTSSK2−/− male flies (n = 10 per group). Data are mean ± SEM. c Live imaging of testes and seminal vesicles of the indicated genotypes. Sperm nuclei (green) are labeled with protamine GFP-Mst35Bb. Cytological examination showing defects of whole testes in dTSSK2−/− male flies. Scale bar, 100 μm. d Quantification of tail-beat frequency of sperm by wild-type (w1118), P{dTSSK2K105M}; dTSSK2−/− and P{dTSSK2T237A}; dTSSK2−/− males (n = 30 per group). Data are mean ± SEM.

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