Fig. 3: Expression of Ca2+ handling proteins in young and adult MCM-TRPV2-cKO hearts.
From: TRPV2 mediates stress resilience in mouse cardiomyocytes
a Change in localisation of LTCC, NCX1, SERCA, and JP2 in young and adult hearts. Double staining of the antibody of target protein (Green) and DAPI (blue). Scale bar, 100 µm. b–f Representative immunoblots (c) and expression level (d–f) of LTCC, NCX1, SERCA, and JP2 in extracts of young and adult hearts (20 µg per lane), using Caveolin 3 as the internal control on cardiac muscle (N = 3–6 mice per group; see full blots in Supplementary Fig. 4) Data are shown as mean ± standard error of the mean (SEM). *P < 0.05 between indicated groups based on Tukey–Kramer test. g Representative double staining of phalloidin (red) and DAPI (blue). Scale bar, 100 µm (upper panels). Representative electron micrographs of myofilaments in Floxed-TRPV2 and MCM-TRPV2-cKO hearts. Scale bar, 2 µm (lower panels).
