Fig. 5: Tracking genome signature across distinct genomes.
From: Self-authenticating genomic materials in Escherichia coli via advanced genome signatures
a One fragment of 7035 nt of E. coli Nissle 1917 with genome signature in one gene of iutA. b Nissle iutA fragment was inserted in the genome of E. coli BL21 at different locations (1: 2,035,076; 2: 3,588,753; 3: 3,852,585), E. coli cells cultured on standard LB solid medium scale bar = 1 cm. c These E. coli BL21 strains were sequenced using the PacBio platform, and reads exceeding 2 Kbp in length were directly decoded. Reads with a determined full Nissle fragment are indicated by red dots, while reads with only the determined coded iutA gene but without the full length of the fragment are represented by purple dots. The y-axis indicates the random number of the sequencing read. d Nissle coded iutA fragment (inserting at 18,144,212,270) was successfully determined in a giant genome sequence constructed by jointing all 14 lungfish chromosomes (GCA_016271365.1), a total of 34,557,647,948 nt in length.
