Fig. 4: Three sites in Las17 PPR bind to actin. | Communications Biology

Fig. 4: Three sites in Las17 PPR bind to actin.

From: Competitive binding of actin and SH3 domains at proline-rich regions of Las17/WASP regulates actin polymerisation

Fig. 4

A A representative pyrene assay comparing the impact of Las17 fragment (300–633) with the minimal fragment (342–392) on actin polymerisation (in the absence of Arp2/3). 300 nM each fragment; 3 µM actin. B MST was used to measure binding of different concentrations of Las17 (wild type or with mutations in the actin binding sites (ABS)) to labelled actin. Error bars are standard error of mean. Green trace shows the impacts of all three sites mutagenized; dark blue is with no sites mutagenized.

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