Fig. 9: The effect of las17 P387A mutation on endocytic reporter protein behavior. | Communications Biology

Fig. 9: The effect of las17 P387A mutation on endocytic reporter protein behavior.

From: Competitive binding of actin and SH3 domains at proline-rich regions of Las17/WASP regulates actin polymerisation

Fig. 9

A–C Lifetime of patches from wild type (red) or las17 P387A (blue) expressing cells tagged with fluorescent markers, alongside representative kymographs. A Las17-GFP, (B) GFP-Abp1, (C) Arc-15 mCherry (describing Arp2/3 complex lifetime). Statistical test Unpaired Mann Whitney test, **** indicates p value < 0.0001. A size bar for each set of kymographs is shown on the upper right of each set. For (A) and (C) size bar is 100 nm and for B is 200 nm. Error bars are standard deviation. D Time lapse images showing co-localisation of Las17-GFP (green dots) and Arc15-mCherry (red dots) in wild type cells and las17 P387A cells. The initial las17 P387A images contain two adjacent endocytic sites. The site of interest is labelled in the first two time panels with a white arrow. Exposure 0.5 sec with 1 second time lapse. 120 s recorded. E Quantification of lifetime of Las17-GFP alone (green), Arc15-mCherry alone (red) and overlap between Las17-GFP and Arc15-mCherry (yellow) from multiple endocytic patches is shown (n = 13). Error bars are standard deviation.

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