Fig. 1: PRDM9 (195–415) purification and SPOT peptide array methylation.
From: Investigation and design of the dual specificity of the PRDM9 protein lysine methyltransferase
a Comparison of the amino acid sequences of positions 1–9 of H3K4 and 32–41 of H3K36. The relative position in relation to the correspondent target lysine is indicated as well. b SDS-gel of purified PRDM9 (195–415) stained with Coomassie Brilliant Blue. c Exemplary image of the autoradiography of peptide SPOT array methylation on H3 (1–15) and H3 (29–43) peptides in un-, mono- and dimethylated form by PRDM9 (195–415). Target lysine K-to-A mutant peptides were used as controls. d Exemplary images of the autoradiography of H3K4 and H3K36 specificity scan peptide arrays methylated by PRDM9 (195–415). Arrays of 15 aa long peptides (H3 1–15 and 29–43) were synthesized using the H3K4 and H3K36 template sequences as represented in the horizontal axis. Residue surrounding the target lysine residues were systematically exchanged against other amino acid residues as shown in the vertical axis. See also Supplementary Fig. 2a.
