Fig. 1: Increased ELVAs formation in oocytes and embryos from aged mice.
From: UBE2V1 governs aging induced protein aggregation and developmental defects in oocytes and embryos
A Confocal images of young (7–8 weeks) and old (42–45 weeks) oocytes (GV), and early embryos (2-cell) labeled with Proteostat (red) and counterstained with Hoechst33342 to visualize DNA (blue). B Quantification of the number of the Proteostat compartments. C Confocal images of young and aged mouse oocytes immunolabeled with Proteostat and anti-ubiquitin conjugates FK2. D Colocalization of ubiquitin and Proteostat in the experiment shown in (C). The total number of oocytes analyzed is indicated in the figures. Data were presented as mean ± SD. Statistical analysis was performed using an unpaired two-tailed Student’s t-test for (D), and one-way ANOVA followed by multiple comparisons for (C). A P-value < 0.05 was considered statistically significant.
