Fig. 2: Age-related increase of UBE2V1 level.
From: UBE2V1 governs aging induced protein aggregation and developmental defects in oocytes and embryos
A Western blot analysis of UBE2V1 protein levels in mouse oocytes from different age groups: 7–8 weeks, 9 months, and 12 months. 100 GV oocytes per sample. B Representative images showing UBE2V1 localization in GV oocytes of different age groups. Oocytes from 7 to 8 weeks, 9 months, and 12 months old mice were immunostained for UBE2V1 (green) and stained with Hoechst 33342 for DNA (blue). C Quantitative analysis of UBE2V1 intensity in the nucleus and cytoplasm. D Western blot analysis of UBE2V1 protein levels in young and old 2-cell embryos, 100 embryos per sample. E Representative immunofluorescence images showing UBE2V1 localization in 2-cell embryos from two groups. Embryos were immunostained with anti-UBE2V1 antibody (green) and counterstained with Hoechst 33342 for nuclear DNA visualization (blue). F UBE2V1 intensity profiles along embryo axes (white lines in E) were quantified. The total number of oocytes analyzed is indicated in the figures. Data were presented as mean ± SD.
