Fig. 6: UBE2V1 overexpression mimics the subcellular phenotypes observed in aged oocytes.
From: UBE2V1 governs aging induced protein aggregation and developmental defects in oocytes and embryos
A Schematic diagram of experimental design of subcellular phenotypic analysis. B Confocal images showing mitochondrial distribution in the Young, Old, and Young+OE groups. C Quantification of the proportion of oocytes with clustering mitochondria distribution (arrows). D Confocal images of ROS level in Young, Old, and Young+OE groups. E Quantitative analysis of the ROS fluorescence intensity in Young, old, and Young+OE groups. F Mitochondrial membrane potential in Young, Old, and Young+OE oocytes was evaluated using JC-1 staining. Green fluorescence indicates inactive mitochondria, while red fluorescence indicates active mitochondria in the oocytes. G Histogram showing the JC-1 red/green ratio. H Confocal images of Young, old, and Young+OE oocytes (at 6 h after release for each group) labeled with OPP (conjugated with AF594, red) to indicate new protein synthesis levels, with DNA counterstained using Hoechst 33342 (blue). I Statistical analysis of the OPP fluorescence intensity in Young, Old, and Young+OE. Throughout, data are presented as means ± SD, and one-way ANOVA followed by multiple comparisons was used for statistical analysis. A P-value < 0.05 was considered statistically significant. The total number of oocytes analyzed (from at least three independent experiments) is indicated in each graph.
