Fig. 7: Restoration of UBE2V1 expression alleviates protein aggregation and meiotic defects in aged oocytes.
From: UBE2V1 governs aging induced protein aggregation and developmental defects in oocytes and embryos
A Schematic representation of the UBE2V1 knockdown experiments in old oocytes. B Western blot analysis showing knockdown efficiency of UBE2V1 siRNA. Total proteins from 100 oocytes were loaded in each lane. C Confocal images of Young (7–8 weeks), Old (42–45 weeks), and UBE2V1 knockdown (Old+KD) oocytes at GV stage labeled with Proteostat (red). D Quantification of the number of the Proteostat compartments. E Quantitative analysis of Pb1 extrusion rates in Young, Old, and Old+KD groups. Data were expressed as mean ± SD of at least three independent experiments, and one-way ANOVA followed by multiple comparisons was used for statistical analysis. A P-value < 0.05 was considered statistically significant. Oocytes analyzed in each group was indicated in each figure.
