Fig. 8: UBE2V1 depletion rescues age-related embryonic defects.
From: UBE2V1 governs aging induced protein aggregation and developmental defects in oocytes and embryos
A Schematic diagram showing the rescue experiments in embryos. B Confocal sections of Young, Old, and Old+KD embryos at the 2-cell stages labeled with Proteostat. Proteostat (red), and DNA was counterstained with Hoechst33342 (blue). C Quantification of embryo development rates at E1.5, E2.5, and E4 days from the Young, Old, and Old+KD groups. D Representative images showing LC3 (green) and Hoechst 33342 (blue) staining in the Young, Old, and Old+KD groups. E Mean LC3 fluorescence intensity in the Young, Old, and Old+KD groups. F γH2AX staining was used to assess blastocyst apoptosis in 2-cell embryos derived from the Young, Old, and Old+KD groups. γH2AX (rad) was detected, and DNA was counterstained with Hoechst 33342 (blue). G The mean fluorescence intensity of γH2AX in the Young, Old, and Old+KD was quantified. H Statistical Analysis of Live Birth Rate at E18.5 Following Embryo Implantation. The total number of oocytes analyzed per condition is indicated in each graph. Data were presented as mean ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by multiple comparisons for (E, G, and H), and multiple t-test for (C). A P-value < 0.05 was considered statistically significant.
