Fig. 2: Determination of the crRNA processing proteins in T. thermophilus HB27. | Communications Biology

Fig. 2: Determination of the crRNA processing proteins in T. thermophilus HB27.

From: A type III-associated Cas6 functions as a negative regulator of type I-B CRISPR-Cas system in Thermus thermophilus

Fig. 2

a, b, c The interference activity assay using interference plasmids for different endogenous CRISPR-Cas systems in various T. thermophilus HB27 strains. The interference plasmids carry mini-CRISPR arrays corresponding to different CRISPR-Cas systems, which are designed to target the DNApolyα gene on the T. thermophilus HB27 chromosome (crRNA-DNApolyα). Non-target plasmid was used as control (crRNA-NT). d The cleavage of 5’ FAM-labeled Repeat-1, Repeat-2, and Repeat-3 by Cas6-1 over different time periods. e The cleavage of 5’ FAM-labeled Repeat-1, Repeat-2, and Repeat-3 by Cas6-2 over different time periods. f The cleavage of 5’ FAM-labeled Repeat-1, Repeat-2, and Repeat-3 by Cas5c over different time periods. The ck group indicates that no protein was added to the reaction system. The data show means of three biological replicates. Error bars indicate the standard deviations (SD). Between-group significance was determined using the unpaired two-sample t-test (*P < 0.05, **P < 0.01, and ***P < 0.001). And ns indicates no significance.

Back to article page