Fig. 1: Visualization of the MacAB-TolC pump in the near-to-native E. coli cell envelope. | Communications Biology

Fig. 1: Visualization of the MacAB-TolC pump in the near-to-native E. coli cell envelope.

From: In situ structure and assembly of the ABC-type tripartite pump MacAB-TolC

Fig. 1: Visualization of the MacAB-TolC pump in the near-to-native E. coli cell envelope.The alternative text for this image may have been generated using AI.

a A single Z slice from a tomogram of an E. coli cell expressing MacA, MacB, and TolC. The side view and top view of MacAB-TolC particles are indicated by the red arrowhead and the white arrowhead, respectively. b A tomogram segmentation of (a). All Z slices are shown, including particles in all Z levels within this tomogram. The particle side views are within the cell envelope, while particle top views are located at the top and bottom surface of this cell. c Zoomed-in view of the MacAB-TolC top view particles (indicated by white arrowheads). d Zoomed-in view of the MacAB-TolC side view particles (indicated by white arrowheads). e The 2D projection of the rotationally averaged density map of the in situ structure of MacAB-TolC in the near-to-native (left) and erythromycin-treated (right) E. coli cells. IM inner membrane, OM outer membrane, PG peptidoglycan.

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