Fig. 2: Therapeutic effects of hPMSC transplantation in mice.
A Representative hematoxylin-eosin (H&E) images (5×) of terminal ileum sections of AKR/J mice and SAMP1/Yit mice treated with PBS, 2 × 106 hPMSCs were shown (Scale bar, 500 µm). Zoomed images are at 10× magnification (Scale bar, 250 µm). Muscularis propria, submucosal thickness, and villus height of AKR/J mice, PBS and 2 × 106 hPMSCs-treated SAMP1/Yit mice were measured in five random views per mouse for quantitative analysis. B Representative immunohistochemical images for MPO, Ly6G, S100A8, and S100A9 (10×, scale bar, 250 µm) in each group. The positive areas of MPO, Ly6G, S100A8, and S100A9 expression were measured in five random views per mouse for quantitative analysis. C Gene expression levels of TNF-α, IL-1β, and IL-6 in each group (five mice in each group). D Fluorescence intensity of FITC in serum of each group (five mice in each group). E Representative immunohistochemical images and quantitative analysis for Cldn1 (20×, scale bar, 100 µm), Cldn2 (20×, scale bar, 100 µm), TUNEL (10×, scale bar, 250 µm) and α-SMA (10×, scale bar, 250 µm) in each group. Five fields were randomly selected from five mice for quantitative analysis. Results were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n.s., not significant. Five mice per group were used. After hPMSC transplantation, intestinal inflammation of mice was alleviated, inflammatory cell infiltration was reduced, the number of apoptotic cells was reduced, fibrosis was decreased, and the intestinal barrier was restored.
