Fig. 1: Phenotyping followed by in situ genotyping.
From: Pooled optical screening in bacteria using chromosomally expressed barcodes
A Cartoon highlighting the inserted fluorescent protein ORF (fp) and barcode (BC) in its chromosome context where it replaces the genes lacZ, lacY and lacA. The d0 sequence is added for mRNA stability. B Example of fluorescence images of cells at three different timepoints after switching to chloramphenicol-containing medium. Time point zero indicates swap to medium with chloramphenicol. C Cartoon showing major steps of in situ genotyping. D Fluorescence images of seven rounds of genotyping for the cell traps shown in (B). E Quantified fluorescence density throughout the experiment for one example lineage for the traps highlighted in (B) together with the decoded genotypes from (D). None of the cells in Trap 3 passed the filtering criteria on the cell fluorescence which is indicated by a dashed line at zero.
