Fig. 3: Comparison of cellular contents in G. partita cells between the photoautotrophic and heterotrophic states.

All results are from the photoautotrophic (in the light without glucose) and heterotrophic (in the light with glucose) cultures of wild-type diploid N2. The results shown in the graphs were calculated per cell. A Dry cell weight. B Carbon content. C Nitrogen content. D Total protein content. E SDS-PAGE patterns of total proteins extracted from the same number of photoautotrophic and heterotrophic cells. The positions of Rubisco large subunit (RbcL), small subunit (RbcS), phycocyanin alpha subunit (CpcA), and beta subunit (CpcB) are indicated. F Glycogen content. G Total fatty acid content of all lipids extracted from the cells. H Membrane lipids and TAG contents. Bars represent means ± SD of results from six (A, D), three (B, C, F) or four (G, H) independent cultures, with dots representing individual data points. Asterisks in the graphs indicate statistically significant differences based on Student’s t-test (*P < 0.05; **P < 0.01; ***P < 0.001). The ratio of values in the photoautotrophic state to those in the heterotrophic state is shown above the bars. TAG triacylglycerol, MGDG monogalactosyldiacylglycerol, DGDG digalactosyldiacylglycerol, SQDG sulfoquinovosyldiacylglycerol, PC phosphatidylcholine, PE phosphatidylethanolamine, PG phosphatidylglycerol, PI phosphatidylinositol.