Fig. 6: Schematic comparison of protein and metabolite abundances in the glycolytic/gluconeogenic and lipid biosynthetic pathways between the photoautotrophic and heterotrophic states.

The results are from the photoautotrophic (in the light without glucose) and heterotrophic (in the light with glucose) cultures of wild-type diploid N2. The metabolic pathways, subcellular compartments (plastid, mitochondria, endoplasmic reticulum [ER], and cytosol), and abbreviated protein names are primarily based on previous studies^90,91 (see Supplementary Data 6 and 9 for detail). Square colors indicate the log₂ fold change in protein abundance from DIA proteome analysis (Supplementary Data 6, 9, and 10), while rounded square colors indicate the log₂-transformed metabolite ratios from GC-MS metabolome analysis (Supplementary Fig. 10; Supplementary Data 3) and lipid analysis (Fig. 3H) in wild-type diploid N2, according to the color key at the top of the figure. Metabolites that were not the targets of metabolome analysis are not surrounded by rounded squares. ‘Not detected’ indicates that the protein level was below the detection limit in the analysis. ‘Not identified’ indicates that the protein-encoding gene was not identified in the G. partita genome. The transport system of fatty acids from ER to plastid is largely unknown⁹¹ (dashed arrow). 1,3BPG 1,3-bisphosphoglycerate, 2PGA 2-phosphoglycerate, 3PGA 3-phosphoglycerate, ACP acyl carrier protein, CDP-DAG CDP-diacylglycerol, DAG diacylglycerol, DGDG digalactosyldiacylglycerol, DHAP dihydroxyacetone phosphate, F1,6BP fructose 1,6-bisphosphate, F6P fructose 6-phosphate, G3P glycerol 3-phosphate, G6P glucose 6-phosphate, GAP glyceraldehyde 3-phosphate, LPA lysophosphatidic acid, LPC lysophosphatidylcholine, MGDG monogalactosyldiacylglycerol, OAA oxaloacetate, PA phosphatidic acid, PC phosphatidylcholine, PE phosphatidylethanolamine, PEP phosphoenolpyruvate, PG phosphatidylglycerol, PI phosphatidylinositol, SQDG sulfoquinovosyldiacylglycerol, TAG triacylglycerol.