Fig. 2: CTSB knockout in renal proximal tubular epithelial cells increased renal uric acid excretion.
From: Cathepsin B-dependent glycolysis contributes to reduced renal uric acid excretion in hyperuricemia
a Schematic strategy for the generation of CTSBtecKO mice. b Verification of CTSB in CTSBtecKO mice by western blot. c The levels of SUA, d FEua, e Scr, f U-MALB, g Serum IL-1β, h Serum IL-6 and i Serum TNF-α in different groups of mice. j Western blot of URAT1, ABCG2, and GLUT9 in the kidneys of different groups of mice. Statistical analysis of k URAT1, l ABCG2, and m GLUT9 protein expression. n HE staining of kidneys of different groups of mice. SUA serum uric acid, FEua fractional excretion of uric acid, Scr serum creatinine, U-MALB urine microalbumin. Bars = 25 μm (40×). Bars = 200 μm (5×). n ≥ 3. *P < 0.05, **P < 0.01, **P < 0.001, ****P < 0.0001.
