Fig. 4: Tail and head angle with PTZ. | Communications Biology

Fig. 4: Tail and head angle with PTZ.

From: Automated detection of complex zebrafish seizure behavior at scale

Fig. 4

a Larval schematic showing heading (orange), tail (yellow) and body axis (blue) vectors used to calculate change in tail angle indicated by Ө, measured between tail and body axis vectors. b Representative images of zebrafish larvae displaying examples of different calculated tail angles. c Schematic indicating how heading angle change is calculated. Larva in gray in the previous frame is compared to larva in blue in the current frame and heading angle difference is computed. d Distribution of tail angle measurements displayed at each recording epoch for 3 (left, N = 50.4 million), 5 (center, N = 71 million) and 7 (right, N = 71 million) dpf larvae showing PTZ induces greater tail angles at all ages. e Distribution of heading angle changes with PTZ at all ages.

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