Fig. 6: Seizure activity reflected in inter-eye distance and brain activity.

a Representative inter-eye distance plotted over time for baseline and PTZ TP2 recordings (top) with a seizure event highlighted at higher resolution (bottom). b Outline of a zebrafish overlayed with an image of the HuC:H2B:GCaMP6s reporter expression. Live microscope image of photometry fiber placement with blue shading overlaid to indicate casted light from the probe. Placement of electrode is also shown for simultaneous LFP recording. c Representative baseline recording showing Ca²⁺ trace recorded from probe aligned with LFP showing similar activity. 15 mM PTZ recordings from a larva showing Ca²⁺ trace recorded from probe aligned with LFP showing seizure-like activity in the probe aligning with events LFP. Scale bar represents 50 µm.