Fig. 4: Role of syntenin-1 and HD-PTP in the HAV release from Huh7-A-I-infected cells.

A Effect of HD-PTP and syntenin-1 coding gene silencing on the co-localization of L0 and HP capsids with HD-PTP; at 24 h p.i. capsids were immunolabelled in red, and HD-PTP in green. B Quantitative analysis of the proportion of HAV capsids co-localizing with HD-PTP (M1) in non-silenced (NT-siRNA) and knock-down cells for HD-PTP (siHD-PTP) and syntenin-1 (siSyntenin-1). C Effect of syntenin-1 and HD-PTP coding gene silencing on the co-localization of L0 and HP capsids with syntenin-1; at 24 h p.i. capsids were immunolabelled in red, and syntenin-1 in green. D Quantitative analysis of the proportion of HAV capsids co-localizing with syntenin-1 (M1) in non-silenced (NT-siRNA) and knock-down cells for syntenin-1 (siSyntenin-1) and HD-PTP (siHD-PTP). The confocal images (A, C) layout is as in Fig. 3. The white bar corresponds to 10 µm. Quantitative analyses (B, D) were done using an average of n = 12 images, from n = 2 independent biological replicates. The total number of infected cells counted was in the range of 46-116 and 67-154 for L0 and HP, respectively. Šidák correction was applied in the multiple comparison test. E Effect of HD-PTP and syntenin-1 coding gene silencing on the ratios between the intracellular genomes and the infectious titers in the supernatant, as a measure of HAV release. Dunnett T3 (L0) and Tukey (HP) corrections were applied in the multiple comparison test. Data represent the mean ± standard error from n = 9 independent biological replicates. Each biological replicate included two technical replicates, whose average is depicted. Significant differences are shown.