Fig. 5: Interaction of RAB proteins with HAV capsids.

A Co-localization of HAV capsids and RAB proteins in Huh7-A-I-infected cells. At 72 h p.i., L0 and HP capsids were immunolabelled in red and RAB proteins in green. The confocal images layout is as in Fig. 3. The white bar corresponds to 10 µm. B Quantitative microscopy analysis of the proportion of HAV capsids co-localizing with RAB7A, RAB11A, and RAB35, respectively, was done using n = 14 images, from n = 3 independent biological replicates. The total number of infected cells counted was in the range of 43-48 and 85-124 for L0 and HP, respectively. C Effect of single and double RAB coding genes silencing on the ratios between the intracellular genomes and the infectious titers in the supernatant, as a measure of HAV release. Data represent the mean ± standard error of n = 6 independent biological replicates for RAB7A and RAB11A and n = 12 independent biological replicates in the rest of the experiments. Each biological replicate included two technical replicates, whose average is depicted. Significant differences are shown.