Fig. 6: Vectorial release of HAV from polarized hepatocytes.

A IIF of polarized Huh7-A-I cells using antibodies directed against the Na⁺K⁺ ATPase (green) and ZO-1 (red), markers of the basolateral (BL) and apical (AP) membranes, respectively. Nuclei are blue labeled. Images are in the axial plane. B Same images in the sagittal plane. White bars in (A, B) correspond to 25 µm and 10 µm, respectively. C Permeability of 4 kDa FITC-dextran through the monolayer of polarized Huh7-A-I; data represent the mean ± standard error of n = 4 independent biological replicates. D Mean transcytosis rates estimated at hourly intervals at 37 °C using a MOI of 5. BL-AP: percent of viruses released into the AP chamber after being added to the BL membrane. AP-BL: percent of viruses released into the BL chamber after being added to the AP membrane. Data represent the mean ± standard error of the percentage of input virus from n = 6 independent biological replicates, including each 2 TCID₅₀ titrations, whose average is shown. E Total virus released from the AP and BL membranes in basolateral-infected polarized Huh7-A-I cells. Data represent the mean ± standard error of n = 9 independent biological replicates, including each 2 TCID₅₀ titrations, whose average is shown. Significant differences between the egress through the AP and BL membranes are depicted.