Fig. 3: Cas12 kinetics with monovalent crRNA varying in length.
From: Profiling crRNA architectures for enhanced Cas12 biosensing
a Gel electrophoretic characterization of the transcribed crRNAs. Lane 1 is the DNA ladder and lanes 2–7 are the different crRNAs starting from crRNA-15 to crRNA-40, respectively. b A summary of Cas12a trans-cleavage kinetics with different lengths crRNA at 1 nM target DNA concentration. c Comparison of the average Cas12 trans-cleavage activity for different length crRNAs with (blue bars) and without (orange bars) activator DNA, which was calculated from the slope of the linear portion of the curve in (b). The dot-plot represents individual data points from independent experiments. The signal enhancement (SE, red stars) is plotted on a secondary axis and is calculated from dividing Cas12 activity in the presence of activator by Cas12 background activity in the absence of activator. d Standard curves for each crRNA showing average Cas12 trans-cleavage activity for varying concentrations of activator DNA. Error bars show standard deviation (n = 2).
