Fig. 5: Cas12 SNP selectivity studies.
From: Profiling crRNA architectures for enhanced Cas12 biosensing
A summary of Cas12 SNP sensitivity for each crRNA (a–f). Activities are normalized to WT activity. g A plot showing the average normalized activity for each nucleotide position across all crRNAs. The dot-plot represents individual data points. Error bars show standard deviation (n = 6). The red bars indicate nucleotide positions within the crRNA that were most sensitive to SNP mutations having activities <10% compared to the most active nucleotide position. h A crystal structure showing LbCas12a bound to crRNA and DNA (PDB:5XUZ), where Cas12 is colored green, dsDNA is colored yellow, crRNA is colored gray, and sensitive nucleotide positions in both crRNA and DNA are colored red.
