Fig. 6: Cas12 activity with bivalent crRNA.
From: Profiling crRNA architectures for enhanced Cas12 biosensing
a Gel electrophoresis characterization of bivalent crRNAs. Lane 1 represents the 50 bp DNA ladder, lanes 2–6 represent the different length of bivalent crRNA in the order 142, 147, 152, 157, and 162 ribonucleotides respectively. The gel shows a progressive upward shift as the spacer length of bivalent crRNA increases. b Overall activity of Cas12 with all bivalent crRNAs without dCas9. c Comparison of Cas12 trans-cleavage activity for crRNAs having different spacer lengths with (blue bars) and without (orange bars) activator DNA and in the presence and absence of dCas9. Signal enhancement is shown in red stars and plotted on a secondary axis. d Cas12 activation in the presence of dCas9 with bivalent and monovalent DNA. e Cas12 activation in the presence and absence of dCas9 with bivalent DNA only. f Cas12 activation in the absence of dCas9 with monovalent and bivalent DNA.
