Table 3 Zinc-binding sites in PEDF identified by X-ray crystallography

Site number	Site type	Zinc coordinators				PDB ID	Occupancy	Affected activity ^a
Site number	Site type	Molecule 1	Molecule 2	Molecule 3	H₂O	PDB ID	Occupancy	Affected activity ^a
1	1	Asp44, His299	Glu236	-	+	9J3Q	0.90	Neurotrophic, anti-angiogenic, ECM binding
2	1	Asp44, His299	Glu236	-	+	9J3P	0.90	Neurotrophic, anti-angiogenic, ECM binding
3	1	Asp44, His299	Glu236	-	+	9J3P	0.90	Neurotrophic, anti-angiogenic, ECM binding
4	2	Asp256, Asp258	His381	-	+	9J3P	0.60	ECM binding
5	2	Asp256, Asp258	His381	-	+	9J3P	0.90	ECM binding
6	3	His381	His381	Asp304, Asp300	-	9J3P	0.90	ECM binding
7	4	His105	Glu42 (H₂O)	-	+	9J3P	0.40	Neurotrophic, anti-angiogenic, ECM binding
8	5	Asp256, Asp258 (H₂O)	His381	Asp339	-	9J3P	0.90	ECM binding, neurotrophic, anti-angiogenic

^aSuppression of PEDF functions associated with zinc binding and impeded access to sites responsible for neurotrophic (44-mer fragment 78–121)^61,73,74,75, antiangiogenic (34-mer fragment 44-77)⁸⁵, or ECM-binding (Asp256, Asp258 and Asp300)⁸⁶ activities.

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