Fig. 4: IL-6 and G-CSF drive emergency myelopoiesis during severe influenza infection in vivo. | Communications Biology

Fig. 4: IL-6 and G-CSF drive emergency myelopoiesis during severe influenza infection in vivo.

From: Systemic cytokines drive conserved severity-associated myeloid responses across bacterial and viral infections

Fig. 4

a Schematic of the influenza dose titration experiment (n = 5 mice for each dose and timepoint). Figure created with Biorender.com (b) IL1R2 surface expression at day 7 post-infection in monocytes (left) and neutrophils (right) in the blood of mice infected with the indicated PR8 titer. c Number and d fraction of IL1R2+ blood monocytes in mice infected with PR8 at the indicated time point. e Number and f IL1R2 mean fluorescence intensity (MFI) of blood neutrophils in mice infected with PR8 at the indicated time point. g Schematic of the IL6 and G-CSF blockade experiment (n = 5 for mock infection, n = 10 mice for infected and antibody-treated groups). h Number of total monocytes, i IL1R2+ monocytes, j neutrophils, and k neutrophil IL1R2 mean fluorescence intensity (MFI) at day 7 post-infection in the BALF of mice infected with 104 pfu PR8 and treated with the indicated antibodies. l cfDNA and m sRAGE levels at day 7 post-infection in the BALF of mice infected with 104 PR8 and treated with the indicated antibodies. Asterisks indicate a significant difference (FDR < 0.05, Mann-Whitney test with Benjamini-Hochberg correction for multiple comparisons). BALF bronchoalveolar lavage fluid, Iso isotype control.

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