Fig. 4: Mutants for the NE healing complex ESCRT-III partly phenocopy Δmsc1.
From: Continuous nuclear envelope surveillance is required for DNA double strand break repair
a Spot assay against phleomycin of ESCRT-III mutants. The HR mutant Δrad52 and Δmsc1 were included as references. Note that the Δsnf7 (the core component of ESCRT-III) is more sensitive to DSBs than the others, whereas Δchm7 (ESCRT-III recruiter to NE) is not. b Phle:mock ratios of retrograde chromatin bridges in cdc15-2 (WT) and ESCRT-III-deficient late-M cells (mean ± s.e.m., n = 3). c Abnormal late-M NE morphologies in ESCRT-III mutants (mean ± s.e.m., n = 3). Four categories were assessed based on Sec61-eYFP: a, normal hourglass shape; b, partitioned; c, blurred; d, multivesiculated. The strain also carries the Nup49-eCFP construct. d Representative micrographs of the four categories. All strains, including those labeled as “WT”, harbor the cdc15-2 allele. Scale bars correspond to 3 μm; BF, bright field. The unpaired t test was used for statistical comparisons (**** for p < 0.0001, ** for p < 0.01).
