Table 1 Strains used in this work

From: Continuous nuclear envelope surveillance is required for DNA double strand break repair

Name1

Genotype2

Origin

SLJ1838

MATα can1Δ::STE2p:SpHIS5 lyp1Δ his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 LYS2

Jaspersen lab

SLJ7465

MATα can1Δ::STE2pr-SpHIS5 lyp1Δ his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 LYS2; [NOP1p:GFP11:mCherry:SCS2TM::LEU2 (pSJ1568)]

Jaspersen lab

SLJ7859

MATα can1Δ::STE2p:SpHIS5 lyp1Δ his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 LYS2; [NOP1p:GFP11:mCherry:PUS1::LEU2 (pSJ1321)]

Jaspersen lab

SLJ8344

MATα can1Δ::STE2p:SpHIS5 lyp1Δ his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 LYS2; [NOP1p:mCherry:SCS2TM:GFP11::LEU2 (pSJ1602)]

Jaspersen lab

FM2995

SLJ7859; MSC1:yGFP1–10::CaURA3MX

This study

FM2996

SLJ1838; MSC1:yGFP1–10::CaURA3MX

This study

FM2997

SLJ8344; MSC1:yGFP1–10::CaURA3MX

This study

FM2999

SLJ7465; MSC1:yGFP1–10::CaURA3MX

This study

AS499*

MATa bar1∆ leu2-3,112 ura3-52 his3-∆200 trp1-∆63 ade2-1 lys2-801

Strunnikov lab

FM2531*

AS499; cdc15-2:9myc::Hph; HMLα ∆hmr::HIS3MX; leu-2-3::lexOp:HO:FLAG:CYC1t::ACT1p:LexA-ER-B112:CYC1t::LEU2MX (pRG646)

Machín lab

FM2942

FM2531; SEC61:eYFP::KanMX

This study

FM2974

FM2531; Δmsc1::NatNT2; SEC61:eYFP::KanMX

This study

FM588

AS499; ade2-1::TetR:YFP::ADE2; tetO(5.6Kb)::1061Kb-ChrXII::HIS3; cdc15-2:9myc::HphNT1

Machín lab

FM2976

FM588; NUP49:mCherry::KanMX6; Δmsc1::NatNT2; SEC61:CFP::TRP1

This study

FM2831

FM2531; MSC1:eYFP::KanMX

This study

FM3149

FM2942; NUP49-eCFP::TRP1

This study

FM2807

FM588; Δmsc1::NatNT2

This study

FM2354

AS499; ade2-1::TetR:YFP::ADE2; tetO(5.6Kb)::194Kb-ChrXII::HIS3; cdc15-2:9myc::HphNT1; HTA2:mCHERRY::KanMX6

Machín lab

FM3127

FM2354; Δmsc1::NatNT2

This study

FM82

AS499; Δrad52::kanMX4

Machín lab

FM3139

FM588; Δdid4::NatNT2

This study

FM3142

FM588; Δsnf7::NatNT2

This study

FM3145

FM588; Δvps24::NatNT2

This study

FM3247

FM588; Δchm7::NatNT2

This study

FM3140

FM2354; Δdid4::NatNT2

This study

FM3144

FM2354; Δsnf7::NatNT2

This study

FM3147

FM2354; Δvps24::NatNT2

This study

FM3251

FM2354; Δchm7::NatNT2

This study

FM3129

FM2942; Δdid4::NatNT2

This study

FM3130

FM2942; ΔSnf7::NatNT2

This study

FM3131

FM2942; ΔVps24::NatNT2

This study

FM3151

FM3129; NUP49-eCFP::TRP1

This study

FM3152

FM3130; NUP49-eCFP::TRP1

This study

FM3162

FM3131; NUP49-eCFP::TRP1

This study

FM3252

FM3149; Δchm7::NatNT2

This study

FM3254

FM2807; Δchm7::KanMX

This study

FM3259

FM2807; Δsnf7::KanMX

This study

FM3258

FM2807; Δdid4::KanMX

This study

FM3263

FM2807; Δvps24::KanMX

This study

FM3281

FM3127; Δsnf7::TRP1

This study

FM3298

FM2976; Δsnf7::URA3

This study

FM2956

FM2531; Δmsc1::NatNT2; RAD52:mCherry::KanMX; NUP49:eCFP::TRP1

This study

FM2878

FM2831; RAD52:mCherry::KanMX

This study

FM3285

FM2878; Δchm7::TRP1

This study

FM3274

FM2878; Δsnf7::TRP1

This study

FM3001

SLJ7465; CDC42p:KAR2SS:GFP1-10:MSC1::NatMX

This study

FM3006

SLJ8344; CDC42p:KAR2SS:GFP1-10:MSC1::NatMX

This study

FM3009

SLJ7859; CDC42p:KAR2SS:GFP1-10:MSC1::NatMX

This study

FM3007

SLJ1838; CDC42p:KAR2SS:GFP1-10:MSC1::NatMX

This study

FM2947

FM2531; RAD52:mCherry::NatNT2; NUP49:eGFP::TRP1

This study

FM2954

FM2531; Δmsc1::NatNT2; RAD52:mCherry::KanMX; NUP49:eGFP::TRP1

This study

FM2381

AS499; GFP:TUB1::URA3; cdc15-2:9myc::HphNT1

F. Machín lab

FM2789

FM2381; Δmsc1::NatNT2

This Study

FM2317

AS499; ade2-1::TetR:YFP::ADE2; tetO(5.6Kb)::194Kb-ChrXII::HIS3; cdc15-2:9myc::HphNT1; CIN8:mCherry::KanMX6

Machín lab

FM2804

FM2317; Δmsc1::NatNT2

This study

BY4741

MATa his3∆1 leu2∆0 met15∆0 ura3∆0

Euroscarf

Y00540

BY4741; ∆rad52::kanMX4

Euroscarf

FM3002

BY4741; Δdid4::HphNT1

This study

FM3004

BY4741; Δsnf7::NatNT2

This study

FM3003

BY4741; Δvps24::NatNT2

This study

FM2865

MATα his3∆1 leu2∆0 lys2∆0 ura3∆0; Δmsc1::NatNT2

This study

FM1391

Mata ura3-52::ADH1p:OsTIR1:9myc::URA3 ade2-1 his3-11,15 leu2-3,112 trp1-1 can1-100; NET1:GFP::LEU2; CDC14:AID*::KanMX

Machín lab

FM3170

FM588; SNF7:AID*:9myc::KanMX

This study

FM3188

FM3170; ura3-52::ADH1p:OsTIR1:9myc::URA3

This study

FM3172

FM2807; SNF7:AID*:9myc::KanMX

This study

FM3189

FM3172; ura3-52::ADH1p:OsTIR1:9myc::URA3

This study

FM3173

FM588; VPS24:AID*:9myc::KanMX

This study

FM 3175

FM2807; VPS24:AID*:9myc::KanMX

This study

FM3190

FM3173; ura3-52::ADH1p:OsTIR1:9myc::URA3

This study

FM3191

FM3175; ura3-52::ADH1p:OsTIR1:9myc::URA3

This study

FM3166

FM588; DID4:AID*:9myc::KanMX

This study

FM3168

FM2807; DID4:AID*:9myc::KanMX

This study

FM3186

FM3166; ADH1p:OsTIR1-9myc::URA3

This study

FM3187

FM3168; ADH1:OsTIR1-9myc::URA3

This study

FM3198

FM3189; HTA2:eCFP::TRP1

This study

FM3202

FM3189; SEC61:eCFP::TRP1

This study

FM3200

FM3189; NUP49:eCFP::TRP1

This study

FM3184

AS499; NUP49:mCherry::NatNT2; HTA2:eCFP::TRP1

This study

FM3238

FM3184; SEC61:eYFP::KanMX; Δsnf7::URA

This study

FM3194

FM3184; SNF7::eYFP::HphNT1

This study

FM3246

FM3184; SNF7:6HA::HIS3

This study

  1. 1The order of the strains is as they first appear in the main figures, then in the Supplementary Figs. The correspondence between the strains and the figures can be found in Table S1. The asterisk (*) indicates that the strain was not used in the figures, but is parental to others, thus simplifying genotype annotations below.
  2. 2Semicolon (“;”) separates genetic modifications accomplished sequentially through transformation. Intermediate strains are omitted. A non-italic beginning refers to a parental strain above.
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