Fig. 3: Imaging unit calibration for pH level detection using a fluorescent dye with a wound proxy. | Communications Biology

Fig. 3: Imaging unit calibration for pH level detection using a fluorescent dye with a wound proxy.

From: A modular fluorescent camera unit for wound imaging

Fig. 3

a Calibration setup diagram using the imaging unit face down and positioned atop a polydimethylsiloxane (PDMS) wound proxy. The fluorescent sample is pipetted into a well that simulates the wound bed within the PDMS proxy. The ribbon cables are not pictured. b Calibration results for Snarf, a pH-sensitive fluorescent dye, buffered from pH 5 to 9. The pH is shown on the x-axis, and the fluorescent intensity, represented by the red color channel, is shown on the y-axis. The line represents the calibrated linear model for pH detection using the mean fluorescent intensity, indicated by red dots. The distribution of the fluorescent intensity in each cropped image (n = 858,407 pixels) is shown as box plots, where the center line denotes the median, the box spans the interquartile range (IQR), and the whiskers extend to the most extreme data points within 1.5 × IQR. c Cropped images from the imaging unit at each pH level used for the calibration.

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