Fig. 1: TAM inhibits ferroptosis to induce cisplatin resistance in GC. | Communications Biology

Fig. 1: TAM inhibits ferroptosis to induce cisplatin resistance in GC.

From: Exo-miR-1911-5p regulates ferroptosis to promote macrophages M2 polarization-mediated gastric cancer cisplatin resistance via MYB/AKR1B10/ACC

Fig. 1

A Validation of macrophage polarization: Protein levels of CD206, CD163, ARG1, iNOS in TAMs from human GC tissues vs. NTAMs from normal tissues by Western blot. β-actin served as a loading control (n  =  3); B–F Co-culture with TAMs/NTAMs in the presence or absence of Fer-1 to investigate DDP-induced malignant transformation via (B: CCK-8, C, D: Colony formation, E, F: EdU) (n  =  3); G Relative viability of GC cells co-cultured with TAMs/NTAMs exposed to cisplatin at the indicated concentrations (n  =  3); H The IC50 of GC cells treated with cisplatin (n  =  3); I Relative viability of GC cells co-cultured with TAMs/NTAMs exposed to Erastin at the indicated concentrations (n  =  3). J The IC50 of GC cells treated with Erastin; K–M Co-culture with TAMs/NTAMs in the presence or absence of Fer-1 to investigate Erastin-induced malignant transformation via (K: CCK-8, L: Colony formation, M: EdU) (n  =  3). Representative of at least 3 experiments, data displayed as mean ± SD. ****p  <  0.0001, ***p  <  0.001, **p  <  0.01, *p  <  0.05.

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