Fig. 5: Exo-miR-1911-5p/MYB/AKR1B10 regulates ferroptosis through ACC pathway. | Communications Biology

Fig. 5: Exo-miR-1911-5p/MYB/AKR1B10 regulates ferroptosis through ACC pathway.

From: Exo-miR-1911-5p regulates ferroptosis to promote macrophages M2 polarization-mediated gastric cancer cisplatin resistance via MYB/AKR1B10/ACC

Fig. 5

A Cell viability after DDP stimulation detected by CCK-8 after MYB knockdown (n  =  3); B, C Lipid peroxidation levels were detected after MYB knockdown (B Lipid ROS; C MDA) (n  =  3); D Western blot was performed to detect protein levels of MYB and AKR1B10 after MYB knockdown (n  =  3). E Detection of the GSH/GSSG ratio after knocking down MYB (n  =  3); F Subcellular localization of AKR1B10 and ACC in GC cell lines; G, H The binding of AKR1B10 to ACC was verified by COIP experiments. I The expression of ACC protein were detected after exosomes addition (n  =  3). J ACC activities in GC cells exposed to DDP treated with exo-mimics/inhibitor (n  =  3); K ACC activities in GC cells exposed to DDP treated with exo-inhibitor/si-MYB (n  =  3); L–N Phenotypic changes in ferroptosis after the addition of ACC inhibitors (L lipid ROS; M. MDA; N:GSH/GSSG) (n  =  3). Representative of at least 3 experiments, data displayed as mean ± SD. ****p  <  0.0001, ***p  <  0.001, **p  <  0.01, *p  <  0.05.

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