Fig. 6: TTK regulates olaparib resistance through RPA2.
From: TTK activates ATR through RPA2 phosphorylation to promote olaparib resistance in ovarian cancer
OVCAR8 and OV90 cells were transfected with PCMV, TTK, shRPA2 1# or TTK combined with shRPA2 1# for 48 h. A Western blot analysis was performed to detect the levels of TTK, RPA2, and β-actin. B MTT assays were performed to detect the relative cell viability after olaparib treatment. Data are mean ± SD, n = 3, one-way ANOVA. C, D The colony survival assay was conducted to assess the colony formation ability under olaparib treatment. Data are mean ± SD, n = 3, Student’s t-test. E Immunofluorescence was used to evaluate the number of γH2AX, Scale bar: 10 µm. F Representative images of costaining for PCNA (red) and RAD51 (green) foci, Scale bar: 10 µm. G The alkaline comet assay was performed to detect the DNA damage induced by olaparib treatment, Scale bar: 10 µm. (Data are mean ± SD, *P < 0.05, **P < 0.01, Scale bar: 10 µm, n = 3).
