Fig. 6: Competition-cooperation interactions and metabolic relationships across the microbial communities in F-site cold seep water columns.

A The MRO and MIP interactions are analyzed based on different community sizes. MRO (metabolic resource overlap) quantifies competition, the similarity of the nutritional requirements between all species in a community. The smaller the MRO, the more complementary the microbial metabolisms are to each other. MIP (metabolic interaction potential) quantifies cooperation, the number of metabolites that can be exchanged among the community members to decrease their dependency on the abiotic environment. Alluvial diagrams show metabolic compounds exchanged between main MAGs in surface (B), mesopelagic (C), and BWI (D) of the cold seep water columns. The left column on the diagram represents the metabolic substrate donor orders, and the right column represents the receiver orders. The colors are used only to distinguish the distinct components of the alluvial diagrams.