Fig. 5: Screening and validation of candidate genes. | Communications Biology

Fig. 5: Screening and validation of candidate genes.

From: Agxt2l-mediated glycerophospholipid metabolism in trophocytes explains Apis mellifera queen’s higher oviposition over A. cerana

Fig. 5: Screening and validation of candidate genes.

a The 49 genes that contain a differential peak in their promoter region. b–e Bees ovaries in the control group and RNAi agxt2l, spr and nedd8. f–h agxt2l, spr and nedd8 differed between AmQ and AcQ in all two omics assays. i–k Expression of agxt2l, spr and nedd8 in queen ovaries of both species. l–n Expression of agxt2l, spr and nedd8 in ovaries in RNAi injected queens and control. o–t Body weight and number of ovarioles in A. mellifera injected with siRNA of agxt2l, spr, nedd8 and the control. u The glycerophospholipid metabolic pathway. Genes marked in red represent genes that have differences in gene expression be in AmQ vs AcQ. (we determined one side ovary because the other side was used for qRT-PCR. But we presented the total number here by timing it by 2 to be consistent with Fig. 1f).

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