Fig. 3: The effect of social isolation on exploration.

A Experimental design: Zebrafish and paradise fish of late larval stage (27–30 dpf) were kept in isolation or in social groups for 4 consecutive days and their behaviour was monitored with the SPM and SJ tests. Additional test naïve controls were tested in each testing day to control for carry-over effects of repeated testing. B Body length of the two species in response to social isolation. * on solid line represents significant main effect of the species, * on dashed lines represents significant main effect of social isolation. C centrum enter frequencies in the SPM of the two species in response to social isolation. * on solid line represents significant main effect of the species, * on dashed lines represents significant main effect of social isolation. Mean exploration latency and all enter frequencies of zebrafish (D) and paradise fish (E) in the showjump test. Shallow arm latency and shallow arm enter frequencies of zebrafish (F) and paradise fish (G) in the swimming plus-maze test. Horizontal solid or dashed lines with asterisk represent significant main effect of testing day or significant interaction between testing days and groups, respectively. Vertical dashed lines with asterisk represent significant main effect of the groups. Vertical braces with asterisks or different letters indicate significant post-hoc contrast between groups or testing days, respectively.