Fig. 8: GRP75 deficiency alleviates RPE dysfunction and AMD progression in vivo. | Communications Biology

Fig. 8: GRP75 deficiency alleviates RPE dysfunction and AMD progression in vivo.

From: Endoplasmic reticulum-mitochondria coupling prompts ZBP1-mediated RPE cell PANoptosis in age-related macular degeneration

Fig. 8: GRP75 deficiency alleviates RPE dysfunction and AMD progression in vivo.The alternative text for this image may have been generated using AI.

A Flowchart of the in vivo experiments. B The H&E staining of mice retinas. White triangle: impaired RPE cells; black asterisk: subsidence of outer nuclear layer (ONL). Scale bars = 50 μm. C Retinal OCT and ultra-wide angle en face images obtained from SS-OCTA showing decreased number of punctate hyperreflective signals (red circle: impaired RPE cells or misalignment of the ONL layer) and thicken ONL by administration of AAV8-shGRP75 in the NaIO3-treated mice. D ERG recordings of mice retinas under scotopic condition. E RPE65 and ZO-1 expressions in mice RPE by immunoblot analysis. Quantification of the protein levels relative to β-actin expression is shown. F, G Immunofluorescent staining of RPE65 and ZO-1 in the indicated groups of mice retinas. Scale bars = 50 μm. (NC: staining without primary antibody). H Immunofluorescent staining of photoreceptor functional indicators (Arrestin, Rhodopsin) in the indicated groups of mice retinas. Scale bars = 50 μm. Data are mean ± SD, the p values were obtained using unpaired t-test (C–E). *p < 0.05 versus AAV8-vector group.

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