Fig. 5: Impact of HEP14-enhanced mitophagy on mitochondrial function to clear ROS. | Communications Biology

Fig. 5: Impact of HEP14-enhanced mitophagy on mitochondrial function to clear ROS.

From: HEP14 treatment improves ovarian function in aged mice through mitophagy enhancement and oxidative stress reduction

Fig. 5

A Representative images and quantification of mitochondrial membrane potential (∆Ψm) levels detected by JC-1 staining (red, high ∆Ψm; green, low ∆Ψm). B Representative fluorescence images and quantification of ROS levels obtained by co-staining with MitoTracker and MitoSox, as well as (C) staining with H2DCFDA. D Protein levels of the key antioxidant genes CAT and TXNL1 by western blotting and densitometric analysis. A–D show the comparison between HEP14-treated senescent KGN cells and vehicle-treated senescent KGN cells coupled with/without mitophagy inhibitor Mdivi-1 and lysosome inhibitor CQ. Data are means ± SEM, n  =  3 in (A–D). P values calculated by one-way ANOVA, followed by Tukey-Kramer test. Experiments were performed three times. Changes were considered statistically significant when P < 0.05. Scale Bar, 100 μm.

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