Fig. 4: D2HDH Activity and stability assays. | Communications Biology

Fig. 4: D2HDH Activity and stability assays.

From: Potassium binding by carbonyl clusters, halophilic adaptation and catalysis of Haloferax mediterranei D-2-hydroxyacid dehydrogenase

Fig. 4: D2HDH Activity and stability assays.

The specific activity of D2HDH as a function of a KCl concentration and b MgCl2 concentration in 1 M KCl, with 2-ketohexanoic acid as the substrate and NADPH (blue) or NADH (orange) as cofactor. c The thermostability (Tm) of D2HDH as a function of KCl (blue) or NaCl (green) concentration and d D2HDH thermostability in 3 M KCl varying MgCl2 concentration. (e) The specific activity of D2HDH in 2 M NaCl (grey) and also with 20% of DMSO (green), glycerol (blue) and ethanol (orange) as a function of 2-ketohexanoic acid concentration. For each graph (a-e) data points (n = 3, except (b) NADH, n = 2) are shown as crosses with the solid line the average for (a-d) and for (e) a Michalis-Menten fitted curve.

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