Fig. 4: Pol IV-mutational hotspots in the P. aeruginosa genome.

A Schematic of the PAO1 chromosome. The circular map displays the localization of the origin (oriC) and replication termination regions, ribosomal RNA clusters (blue arrows), genes (grey lines) and Pol IV-target genes (red lines). The outer and inner bands represent the plus and minus strands, respectively. The black plot shows the percentage GC content plotted as the average for non-overlapping 0.5 kb windows spanning one strand for the whole genome. The genomic map was generated by Proksee⁶⁴. B Mutation rates along the P. aeruginosa chromosome. AT>CG mutation rates were plotted as a function of distance from the origin of replication in 0.25-Mbp bins for the Pol IV-target genes and the mutT (T), mutT mutS^β (TS^β), mutT dinB (TD) and mutT mutS^β dinB (TS^βD) strains. For mutation rate calculations, the number of AT > CG per bin was divided by the number of AT in coding regions within the bin and generations. Pol IV-target genes were identified from the mutational dataset of the TS^β lines. Mutation rates were estimated as the number of AT>CGs per the number of AT in coding regions in each bin and generations. Error bars show 95% confidence limits. Confidence limits for values fewer than 5 BPS events were not calculated. No overlap of 95% confidence intervals indicates statistically significant differences.