Fig. 1: Phage origin, receptor specificity, and phylogenetic tree of PJNS001 and PJNS002.
From: Structural basis for Salmonella infection by two Microviridae phages
a Schematic representation of LPS structural mutations recognized by PJNS001 and PJNS002. Sugar residues are labeled using standard abbreviations: Man, mannose; Rha, rhamnose; Gal, galactose; Glc, glucose; GlcNAc, N-acetylglucosamine; Hep, L-glycero-D-manno-heptose; Kdo, 2-keto-3-deoxyoctonate; EtNP, ethanolamine phosphate; P, phosphate. Dashed arrows indicate the specific sugar positions added by enzymes encoded by the rfaL, rfaJ, rfaI, and rfaG genes. Hollow arrows indicate the LPS truncation points associated with susceptibility to PJNS001 (ΔrfaL) and PJNS002 (ΔrfaJ), showing that each phage targets distinct regions of the LPS core. b Phage sensitivity assay for PJNS001 and PJNS002 with different mutations. Infection specificity of phages PJNS001 and PJNS002 toward distinct receptor-deficient strains was determined via spotting assays. c Phylogenetic tree of PJNS001 and PJNS002 (red star). Phylogenetic analysis was performed using VipTree software. A total of 155 phages representing distinct viral families were analyzed, including Inoviridae, Microviridae, Plectroviridae, Pleolipoviridae, and Paulinoviridae.
